Construction and analysis of a subtracted cDNA library of Betula platyphylla female inflorescence

编号 zgly0000361546

文献类型 期刊论文

文献题名 Construction and analysis of a subtracted cDNA library of Betula platyphylla female inflorescence

学科分类 220.99;林学其他学科

作者 WEIJi-cheng  YANGChuan-ping  WANGChao  JIANGJing 

作者单位 ForestryCollege  NortheastForestryUniversity  Harbin150040  P.R.China  ForestryCollege  NortheastForestryUniversity  Harbin150040  P.R.China//MudanjiangTeachersCollege  Mudanjiang157012  PR.China 

母体文献 Journal of Forestry Research;林业研究: 英文版 

年卷期 2005,16(2)

页码 97-100

年份 2005 

分类号 S792.153 

关键词 白桦  雌花序  cDNA文库  SMART  RT-PCR  基因表达 

文摘内容 Female inflorescence of Betula platyphylla was sampled at an interval of each two days to analyze the background of gene expression in floral phase. On the basis of SMART strategy, the driver cDNA was obtained from total RNA of the last sample and the tester cDNA was from that of the others by RT-PCR which were subsequently used to construct a subtracted cDNA library. The result of the ESTs (expression sequence tags) blastX showed that the genes in the subtracted cDNA library could be mainly clustered into 5 groups related to metabolism, transportation and signal transduction, cell cycle, stress response, and regulation. The relationship between gene expression and development was also discussed。