Cis- and Trans-Cinnamic Acids Have Different Effects on the Catalytic Properties of Arabidopsis Phenylalanine Ammonia Lyases PAL1, PAL2, and PAL4

编号 zgly0000370050

文献类型 期刊论文

文献题名 Cis- and Trans-Cinnamic Acids Have Different Effects on the Catalytic Properties of Arabidopsis Phenylalanine Ammonia Lyases PAL1, PAL2, and PAL4

学科分类 220.1070;森林植物学

作者 Ming-JieCHEN  VeerappanVIJAYKUMAR  Bing-WenLU  BingXIA  NingLI 

作者单位 DepartmentofBiology  InstituteofBotanyofJiangsuProvinceandtheChineseAcademyofSciences 

母体文献 Journal of Integrative Plant Biology;植物学报: 英文版 

年卷期 2005,47(1)

页码 67-75

年份 2005 

分类号 Q949.4 

关键词 肉桂酸  酶动力学  苯丙氨酸解氨酶  催化反应  拟南芥属 

文摘内容 Cis-cinnamic acid (CA) is a naturally occurring compound, presumably converted from transCA in higher plants. To investigate the effect of cis-CA on the activity of Arabidopsis phenylalanine ammonia lyase (PAL), AtPAL1, AtPAL2, and AtPAL4 genes were isolated using reverse transcription polymerase chain reaction. These genes were fused to a glutathione S-transferase gene and overexpressed in a heterologous prokaryotic system of Escherichia coli. The purified PAL1, PAL2 and PAL4 enzymes were characterized biochemically to determine the effects of cis-CA on the kinetic parameter Km. The results showed that cis-CA is a competitive inhibitor for PAL1, but not PAL2 and PAL,4, whereas trans-CA acts as a competitive inhibitor for all three PAL isomers, suggesting that cis- and trans-CA have different effects on the catalytic activity of PAL。