Cloning and Analysis of Telomere-associated Sequences of Ginkgo biloba L.

编号 zgly0000362555

文献类型 期刊论文

文献题名 Cloning and Analysis of Telomere-associated Sequences of Ginkgo biloba L.

学科分类 220.99;林学其他学科

作者 LiuDi  LuHai  JiFei-teng  LiFeng-lan  GuoHui-hong 

作者单位 CollegeofBiologicalSciencesandBiotechnology  BeijingForestryUniversity  Beijing100083  ER.China 

母体文献 Forestry Studies in China;中国林学: 英文版 

年卷期 2005,7(1)

页码 7-10

年份 2005 

分类号 S792.95 

关键词 基因克隆  序列分析  端粒  银杏  PCR  Ginkgo  biloba  L.  阿布属 

文摘内容 Total genomic DNA was extracted from leaves of Ginkgo biloba L. by the method of hot CTAB. After determining quantification of DNA sample by microclorimetric spectrophotography, Arabidopsis-type telomere primer and Sau3A I cassette primer were used to isolate telomere-associated sequences from G. biloba L. by the method of cassette-ligation-mediated polymerase chain reaction (PCR). Using this method, two telomere-associated sequences, TAS1 and TAS2, were isolated. The authors preformed Southern hybridization ofEcoR I-treated G. biloba genomic DNA with each clone. The hybridization pattern showed that the clones obtained were derived from G. biloba genomic DNA. There are the Arabidopsis-type TTTAGG tandem repeats in telomeres of G.biloba。