Optimization of Cultivation Conditions for Exopolysaccharide and Mycelial Biomass by Cfitocybe sp. Using Box-Behnken Design

编号 zgly0000347952

文献类型 期刊论文

文献题名 Optimization of Cultivation Conditions for Exopolysaccharide and Mycelial Biomass by Cfitocybe sp. Using Box-Behnken Design

作者 WANGYun-xiang  LUEFeng-xia  LUZhao-xin 

作者单位 LaboratoryofFoodProcessingandQualityControl 

母体文献 Agricultural Sciences in China;中国农业科学: 英文版 

年卷期 2004,3(9)

页码 687-694

年份 2004 

分类号 S646.01 

关键词 响应曲面  RSM  栽培条件  菌丝体  生物量  杯伞属  真菌 

文摘内容 Response surface (RSM) methodology based on a three-level three-factor Box-Behnken design of experiment was used to optimize the exopolysaccharide content (EPC) and the mycelium biomass in submerged cultivation by Clitocybe sp. AS 5.112. The critical factors selected for the investigation were cultivation temperature, time and volume of medium, based on the results of previous Plackett-Burman design. By analyzing the response surface plots, the optimum ranges of cultivation temperature, time and medium volume for obtaining over 1 253.00μg mL^-1 of EPC lie in 24.3-25.8℃, 9.7-10.2d and 76.0- 90.0 mL, respectively. While for obtaining over 8.32 mg mL^-1 of dry cell weight (DCW), the above variables would be in the range of 23.8-24.8℃, 9.6- 10.3 d and 71.0- 98.0mL,respectively. By solving the inverse matrix from the quadratic regression equations, the optimal conditions to gain 1 265.45μg mL^-1 of EPC were 25.0℃, 9.9d and 83.4mL, to gain 8.50mg mL^-1 of DCW were 24.4℃, 9.9d and 87.1mL. In order to obtain the maximum yield of EPC and DCW at the same time, the above conditions would be 24.5℃, 9.9 d and 84.7 mL,respectively, in this situation, the maximum predicted EPC and DCW were 1 261.60μg mL^-1 and 8.47mg mL^-1, respectively. The experimental data under various conditions have validated the theoretical values。