CTAB-silica Method for DNA Extraction and Purification from Castanea mollissima and Ginkgo biloba

编号 zgly0000301316

文献类型 期刊论文

文献题名 CTAB-silica Method for DNA Extraction and Purification from Castanea mollissima and Ginkgo biloba

学科分类 220.99;林学其他学科

作者 ShenYongbao  ShiJisen 

作者单位 NationalSouthernTreeSeedInspectionCenter 

母体文献 中国林学: 英文版 

年卷期 2003,5(3)

页码 10-12

年份 2003 

分类号 S792.95  S664.2 

关键词 银杏  栗  DNA  提取  纯化  溴化十六烷三甲基铵-硅酸法 

文摘内容 A new method CTAB-silica for DNA extraction and purification from the leaves and buds of Castanea mollissima and Ginkgo biloba was tested. The method is based on the silica-based purification protocol developed by Boom et al. (1990). By modifying the protocol, plant genome DNA could be extracted easily from dormant buds, mature leaves, and other parts of plant. Our results showed that the purified DNA was of high purity and could be analyzed by PCR. Furthermore, this CTAB-silica method took much less time for a successful DNA purification process compared to the traditional methods (CTAB and SDS). By our method,the suitable DNA can be extracted and purified from over 10 plant samples by one person in an hour。