长裙竹荪凝集素的分离纯化与部分生化性质

编号 zgly0000305755

文献类型 期刊论文

文献题名 长裙竹荪凝集素的分离纯化与部分生化性质

作者 林玉满  苏爱华 

作者单位 福建师范大学化学系 

母体文献 中国生物化学与分子生物学报 

年卷期 2003,19(2)

页码 261-263

年份 2003 

分类号 Q949.329  Q946.1 

关键词 长裙竹荪凝集素  分离  纯化  生化性质 

文摘内容 The lectin from Dictyophora indusiata Fisch was isolated and purified by physiological saline extraction,(NH4)2SO4 precipitation and Sephadex G-75 ,Sepharose 4B chromatography,It turned out to be a single band on PAGE.SDS-PAGE showed that it contained one subunit ,whose molecular mass was 53000. This lectin agglutinated rabbit erythrocytes and human erythrocytes,it also agglutinated the peripheral lymphocytes cells and S180 tumor cells from mouse,The hemagglutination of the lectin on rabbit erythrocytes was inhibited by lactose and L-fucose among the sugar tested,Amino acid composition analysis showed that it contained 15 kinds of amino acid ,in which the content of aspartic acid and valine was relatively high。