Transcriptome-wide identification of WRKY family genes and their expression under cold acclimation in Eucalyptus globulus

编号 040019005

推送时间 20190610

研究领域 森林培育 

年份 2019 

类型 期刊 

语种 英语

标题 Transcriptome-wide identification of WRKY family genes and their expression under cold acclimation in Eucalyptus globulus

来源期刊 trees

期 第190期

发表时间 20190504

关键词 RNA-Seq;  Abiotic stress;  Cold stress;  Transcription factors; 

摘要 The WRKY transcription factor family is involved in multiple biological processes, especially in the transcriptional regulation associated with plant abiotic stress response. This gene family has not been previously characterized in Eucalyptus globulus, a sensitive species to freezing temperatures, able to develop a certain degree of frost tolerance when exposed to low but nonfreezing temperatures, a phenomenon known as cold acclimation. In this study, based on the RNA-seq data from cold-acclimated E. globulus plants, a total of 51 EglWRKY genes were identified. Phylogenetic analysis allowed to classify these genes into three main groups and five subgroups, according to their putative orthologous genes in Arabidopsis. The expression patterns of 51 EglWRKY genes under cold stress were determined by in silico DEG analysis in leaf and root. Transcriptome profiling indicated that the expression of multiple EglWRKY genes is activated under chilling (4 °C) and freezing temperatures (? 6 °C). Expression patterns derived from qRT-PCR showed that 11 EglWRKY genes are regulated during cold acclimation in leaf tissue. These results provide the basis for further studies and analysis of WRKY genes to identify their function and molecular mechanisms involved in plant abiotic stress responses in E. globulus.

服务人员 孙小满

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