基于二代测序的集群分离分析法在木本植物基因定位中的应用

编号 lyqk008179

中文标题 基于二代测序的集群分离分析法在木本植物基因定位中的应用

作者 梁晓涵  刘婷婷  张烨  程堂仁  王佳  张启翔  潘会堂 

作者单位 花卉种质创新与分子育种北京市重点实验室, 国家花卉工程技术研究中心, 城乡生态环境北京实验室, 北京林业大学园林学院, 北京 100083

期刊名称 世界林业研究 

年份 2020 

卷号 33

期号 2

栏目编号 1

栏目名称 专题论述 

中文摘要 植物重要性状相关的数量性状基因座（QTL）和基因定位一直是结构基因组学的研究重点，也是遗传机制解析和分子育种的重要基础。木本植物具有重要的经济、生态价值，但用于其研究的传统基因定位方法存在耗时长、工作量大、通量低、成本高且效果不佳等问题。近年来，由于测序技术发展日新月异，基于二代测序技术的集群分离分析法（NGS-based BSA）开始应用于木本植物中。与传统的集群分离分析法（BSA）技术相比，NGS-based BSA周期短、效率高，能对质量或数量性状进行精确定位，在木本植物遗传学和组学研究方面具有广阔的应用前景。文中简述了BSA的发展历程和研究策略，综述了其在木本植物研究中的进展，分析了NGS-based BSA的优势及存在的问题，展望了其在木本植物遗传学、组学和种质改良领域中的应用潜力。

关键词 集群分离分析法  测序技术  数量性状基因座  基因定位  木本植物 

基金项目 北京市科技计划课题“基于组学的北京特色花灌木优良新品种培育及应用”（Z181100002418006）；国家自然科学基金“利用转录组测序技术挖掘调控紫薇株高性状的关键基因”（31470695）。

英文标题 Application of Next-generation Sequencing Based Bulked Segregant Analysis in Gene Mapping of Woody Plants

作者英文名 Liang Xiaohan, Liu Tingting, Zhang Ye, Cheng Tangren, Wang Jia, Zhang Qixiang, Pan Huitang

单位英文名 Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular Breeding;National Engineering Research Center for Floriculture;Beijing Laboratory of Urban and Rural Ecological Environment;School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China

英文摘要 Mapping of functional QTLs and genes relevant to plant traits is always the focus of structural genomics, which also lays a solid foundation for the genetic mechanism deciphering and molecular breeding. Woody plants have significant economic and ecological values. However, the common genetic mapping approaches adopted for woody plant analysis are time-consuming, laboring, low-fluxes, costly and inefficient. Recently, next-generation sequencing-based bulked segregant analysis (BSA) has been used in woody plant QTL and gene mapping with the rapid development of the sequencing technology. Compared with conventional BSA, it has the advantages like short time period, high efficiency and accurate positioning of quality or quantitative traits, which has great potential to be used in the genetics and genomics of woody plants. The paper summarizes the development and strategies of BSA, reviews its latest research progresses in woody plants, analyzes its advantages and extant problems, and prospects its potential to be applied in genetics, genomics and germplasm improvement in woody plants.

英文关键词 BSA;sequencing technology;QTL;gene mapping;woody plant

起始页码 54

截止页码 61

投稿时间 2019-06-11

最后修改时间 2019-11-21

作者简介 梁晓涵,女,硕士研究生,研究方向为花卉分子生物学,E-mail:15501110321@163.com。

通讯作者介绍 潘会堂,男,教授,博士生导师,主要从事园林植物与观赏园艺研究,E-mail:htpan@bjfu.edu.cn。

E-mail 潘会堂，htpan@bjfu.edu.cn

分类号 S718.46

DOI 10.13348/j.cnki.sjlyyj.2019.0114.y

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