Influences of antibiotics on plantlet regeneration via organogenesis in Ioblolly pine (Pinus taeda L.)

编号 zgly0000302945

文献类型 期刊论文

文献题名 Influences of antibiotics on plantlet regeneration via organogenesis in Ioblolly pine (Pinus taeda L.)

学科分类 220.99;林学其他学科

作者 TANGWei  LatoyaHarris  RonaldJ.Newton 

作者单位 DepartmentofBiology 

母体文献 Journal of Forestry Research;林业研究: 英文版 

年卷期 2003,14(3)

页码 185-190

年份 2003 

分类号 S791.255 

关键词 火炬松  抗生素  器官发生  植株再生  愈伤组织  诱导  生长  遗传转化 

文摘内容 Three antibiotics ampicillin, carbenicillin, and cefotaxime were evaluated for their effects on induction, growth, and differentiation of organogenic calli, as well as rooting of regenerated shoots of three Ioblolly pine (Pinus taeda L.) genotypes. Of the antibiotics administered, cefotaxime maximally increased the frequency of callus formation and growth rate of organogeni ccalli, carbenicillin maximally increased the frequency of shoot regeneration and the average number of adventitious shoots per piece of organogenic callus, ampicUlin maximally decreased the rooting frequency of regenerated shoots and mean number of roots per regenerated shoot, in comparison with antibiotic-free media. Compared with the control, ampicillin minimally increased the frequency of callus formation, cefotaxime minimally increased the frequency of shoot regeneration, and carbenicillin minimally decreased the rooting frequency of regenerated shoots in three Ioblolly pine genotypes tested. All three antibiotics increased the frequencies of callus formation and shoot regeneration, and reduced the rooting frequency ot regenerated shoots suggested that the establishment of an efficient Agrobacterium tumefaciens-mediated transformation protocol for stable integration of foreign genes into Ioblolly pine need to select a suitable antibiotic. This investigation could be useful for optimizing genetic transformation of conifers。