利用ISSRDNA标记鉴定主要银杏栽培品种

编号 zgly0000369966

文献类型 期刊论文

文献题名 利用ISSRDNA标记鉴定主要银杏栽培品种

作者 沈永宝  施季森  赵洪亮 

作者单位 南京林业大学国家林业局南方林木种子检验中心  江苏省邳州市林业局 

母体文献 林业科学 

年卷期 2005,41(1)

页码 202-204

年份 2005 

分类号 S565.4  Q948.1 

关键词 ISSR  栽培品种  银杏  利用  RAPD  鉴定  DNA 

文摘内容 Ginkgo biloba is one of most famous officinal plant in China. In the past, many varieties were selected and planted, but there is not still identification system for these varieties. This paper studied DNA fingerprints of the main G. biloba varieties cultivated all over the country using ISSR DNA marker. The results indicated ISSR marker was effective highly for identifying 13 varieties. Only using primer ISSR46 and ISSR44, 13 varieties were able to be identified based on 11 polymorphic loci (ISSR46 - 550, ISSR46 - 670,1SSR46 - 740, ISSR46 - 1000, ISSR46 - 1159, ISSR46 - 1359, ISSR46 - 1600, ISSR44-520,ISSR44 - 580, ISSR44 - 660, ISSR44 - 1750). In addition, we found ISSR marker was stable and repeatable highly compared with RAPD marker。