Comparison of G6PDH Activity and LT50 Between P.tomentosa and P.suaveolens During Freezing Acclimation

编号 zgly0000303647

文献类型 期刊论文

文献题名 Comparison of G6PDH Activity and LT50 Between P.tomentosa and P.suaveolens During Freezing Acclimation

学科分类 220.99;林学其他学科

作者 LinShanzhi  ZhangZhiyi  LiuYuanzhen 

作者单位 CollegeofBiologicalSciencesandBiotechnology 

母体文献 Forestry Studies in China;中国林学: 英文版 

年卷期 2003,5(1)

页码 20-24

年份 2003 

分类号 S792.11 

关键词 杨属  耐冻性  6-磷酸葡萄糖脱氢酶  酶活性  LT50  G6PDH  P.tomentosa  P.suaveolens  作用机理 

文摘内容 The differences of glucose-6P dehydrogenase(G6PDH) activity and freezing resistance induced by freezing acclima-tion between cuttings of freezing-sensitive P.tomentosa and freezing-resistant P.suaveolens were compared for exploring the role of G6PDH on the enhancement of freezing resistance induced by freezing acclimation.After 5d of freezing acclimation at -3℃ ,the LT50 of P.tomentosa has deereased from -6.2℃ incontrol cuttings to -14.3℃ in freezing acclimated ones,and the increase of LT50 of P.tomentosa has decreased from -6.2℃ in control cutting to -14.3℃ in freezing acclimated ones,and the increase of G6PDH activity was observed in freezing acclimated cuttings as compared with control ones.Whereas,when P.suaveolens was freezing acclimated at -20℃ for 5d,the LT50 has decreased from -27.1℃ in control cuttings to -43.5℃ in freezing acclimated ones,and the activity of G6PDH increased considerably.In addition,the increase of LT50 and the decrease of G6PDH activity resulting from 2d of deacclimation at 25℃ were found in two kinds of freezing acclimated cuttings.It is concluded that the increase in the activity of G6PDH may associate with the inherited freezing resistance of species and the enhancemen of freezing resistance of cuttings,and may play an important role in the antifreeze process under freezing temperature,which would provide the basis for the study on the molecular mechanism of freezing resistance in P.suaveolens and the cloning of gene associated with freezing resistance。