Adventitious Shoot Regeneration from Leaf of Uenishiwase Persimmon

编号 zgly0000325342

文献类型 期刊论文

文献题名 Adventitious Shoot Regeneration from Leaf of Uenishiwase Persimmon

作者 MAJun-lian  LIUXiao-na  ZHANGZi-de 

作者单位 CollegeofFoodScienceandTechnology 

母体文献 Agricultural Sciences in China;中国农业科学: 英文版 

年卷期 2004,3(1)

页码 71-74

年份 2004 

分类号 S665.201 

关键词 不定芽  再生  叶子  柿子  品种 

文摘内容 Effects of basal mediums, hormones and their concentrations on the shoot regeneration from leaf of sweet persinmon (Diospyros kaki Thunb. cv. Uenishiwase) were studied by orthogonal design trial. The result showed that modified Murashige and Skoog [MS (1/2 N)] was the most optimum for the regeneration and 1/2 MS was better than MS. Shoot percentage in the medium containing 4.0mg L^-1 ZT(zeatin) was much higher than that of other two concentrations, among which 2.0mg L^-1 ZT was much better than 1.0mg L^-1 ZT and shoot percentage in the concentration of 1.0mg L^-1 ZT was only 4%. There were no any beneficial effects when supplementing IAA in the medium. Shoot percentage and average shoots per explants were dramatically decreased in the 2.0mg L^-1 IAA. Data in the orthogonal trial indicated that ZT was the most effective factor in the shoot regenerating of Uenishwase persimmon and basal medium was important too, but IAA had no any beneficial effects at all. In the orthogonal trial, the best result was achieved in MS (1/2 N) medium containing 4.0mg L^-1 ZT and 1.0mg L^-1 IAA, in which shoot percentage and average shoots per explants were 86% and 2.2, respectively。