人参皂苷Rb2促进牛主动脉内皮细胞纤溶活性

编号 zgly0000315130

文献类型 期刊论文

文献题名 人参皂苷Rb2促进牛主动脉内皮细胞纤溶活性

作者 刘建文  魏东芝 

作者单位 华东理工大学生物化学研究所生物反应器国家重点实验室 

母体文献 Acta Pharmacologica Sinica;中国药理学报: 英文版 

年卷期 2003,24(2)

页码 102-108

年份 2003 

分类号 R284.1  R285.5 

关键词 人参  皂苷类  Rb2  牛  主动脉内皮细胞  纤溶活性  纤维蛋白溶酶活性 

文摘内容 AIM:The effect of ginsenoside Rb2 purified from Panax ginseng on fibrinolytic activity of bovine aortic endothelial cells (BAEC) was in vestigated.METHODS:Cellular plasminogen activator(PA) level of the lysates was measured by the chromogenic substrate S-2403.Fibrin underlay technique was carried out to observe fibrinolysis by growing endothelial cells in the culture medium.Cella viability was the determined by measurement of the activity of mitochondrial dehydrogenase.The ability of Rb2 of potentiating cellular PA activity was investigated by measuring the amounts of PA and PA inhibitor-1(PAI-1) in the culture medium using zymography and reverse zymography.Changes in the expression of urokinase-type PA (uPA),uPA receptor,and PAI-1 mRNA in BAEC atfer treatment with Rb2 were analyzed by Northern blot,RESULTS:Rb2 enhanced cellular PA activity in a concentration-and time-dependent manner.Treatment of BAEC with Rb2 10 mg/L for 9 h resulted in a 3.5-fold increase of PA activity without a marked cytotoxic effect,as shown by LDH levels in culture.Increased PA levels caused the increase in surface plasmin levels as observed by fibrin underlay tecnique Rb2 greatly or moderately increased the amount of urokinase-type PA(uPA) or its inhibitor (PAI-1),present in the culture medium,whereas saponin did not influencce mRNA levels of uPA,its surface receptor,and PAI-1,suggesting that Rb2 may stimulate the secretion of uPA without enhancing its gene expression.The enhancement of PA levels by retinoic acid alone,a stimulator of PA synthesis,was potentiated by the simultaneous addition of ginsenoside Rb2 1 mg/L.Therefore, Rb2 might exert a strong synergism in the synthesis of cellular PA in BAEC.CONCLUSION:Ginsenoside Rb2 enhanced the PA activity levels in BAEC as well as the surface plasmin actvity of BAEC.Rb2 may stimulate the secretion of uPA without enhancing the gene expression of uPA,uPA receptor (uPAR),and PAI-1。