Isolation and Preparative Purification for Ginkgolides A and B

编号 zgly0000315079

文献类型 期刊论文

文献题名 Isolation and Preparative Purification for Ginkgolides A and B

作者 韩金玉  王华  常贺英  褚巧伟 

作者单位 SchoolofChemicalEngineeringandTechnology 

母体文献 Chinese Journal of Chemical Engineering;中国化学工程学报: 英文版 

年卷期 2003,11(2)

页码 125-129

年份 2003 

分类号 R284.2  R284.1 

关键词 纯化  银杏汁  分离  类黄酮  萜烯  提取  分析  生物活性  气相色谱  银杏内酯A  银杏内酯B 

文摘内容 In this paper a simple preparative method for isolation and purification of ginkgolides A and B was developed,As starting material,a commercially available standardized ginkgo extract (EGb761,containing 24% flavonoid and 6% terpene trilactones) was used,After a pretreatment step,optimized by the uniform design method ,the concentrated intermediate extract with high content of GA and gb(+90%) was separated into the individual terpenes by preparative liquid chromatography eluted with petroleum ether-ethylacetate,Analysis of products was carried out by means of HPLC-ELSD(evaporative light -scattering detector),The results show that ginkgolides A and B are obtained in higher yield and better purity。