Embryo culture and rapid propagation of Syringa

编号 zgly0000301203

文献类型 期刊论文

文献题名 Embryo culture and rapid propagation of Syringa

学科分类 220.5010;园林植物学

作者 ZHOULi  DAILi-min  SUBao-ling 

作者单位 InstituteofAppliedEcology 

母体文献 Journal of Forestry Research;林业研究: 英文版 

年卷期 2003,14(3)

页码 213-216

年份 2003 

分类号 S685.26 

关键词 丁香  幼胚培养  离体培养  组织培养  快繁技术  培养基  激素 

文摘内容 Embryo of lilacs (Syringa L) culture in vitro and the rapid propagation were studied. The orthogonal experiments, including the selection of basal medium, embryo age and other factors such as sugar, benzyladenine (BA), naphthalene acetic acid (NAA) and glutamine (Gln), were carried out. The results indicated that the optimal medium for embryo culture was Monnier medium supplemented with NAA (0.001 mg.L^-1), BA (0.1 mg.L^-1), sugar (50 g.L^-1), and Gin (400 mg.L^-1), with a germination rate of 91.7% at least; the optimal embryo age was 50 d; and Gln had significant effects on the germination rate of embryo.Moreover, the optimal medium for subculture was MS+BA (2 mg.L^-1)+NAA (0.001 mg.L^-1)+Gln (0.5 mg.L^-1), with the propagation coefficient of 3.6 at least。