Agrobacterium-mediated Transformation of Rice (Oryza sativa L.) with Atrazine Chlorohydrolase Gene (atzA)

编号 zgly0000372220

文献类型 期刊论文

文献题名 Agrobacterium-mediated Transformation of Rice (Oryza sativa L.) with Atrazine Chlorohydrolase Gene (atzA)

作者 WANGSong-wen  SHILi-li  SUNZong-xiu  CAIBao-li  FUYa-ping  WANGYang  SIHua-min  LIUXia  ZHANGXin 

作者单位 TianjinAgricultralCollege  TianjinAgricultralCollege  KeyLaboratoryforRiceBiology  InstituteofMolecularBiology  KeyLaboratoryforMolecularPhytophathology 

母体文献 Agricultural Sciences in China;中国农业科学: 英文版 

年卷期 2005,4(4)

页码 241-246

年份 2005 

分类号 S511  S451.1 

关键词 转基因水稻  莠去津  氯化羟基水解酶基因  除草剂  土壤杆菌介导 

文摘内容 Atrazine chlorohydrolase gene (atzA) was cloned from Arthrobacter sp. AD1. A plant expression plasmid was constructed under the control of CaMV35s promoter and was used in rice transformation. The target gene was successfully introduced into mature embryos of a japonica rice cultivar Jindao 107 by Agrobacterium- mediated transformation and hundreds of transgenic plants were obtained. The exogenous atzA gene in the transgenic plants that expressed atrazine resistance was confirmed by Southern blot hybridization. The resistance experiments by spraying transgenic rice plants with 0.133% atrazine shown that most of the transgenic rice plants exhibited the resistance to herbicide atrazine. The segregation of exogenous atzA gene in T1 progeny corresponded to the Mendelian ratio。