Ce4+-Induced Apoptosis of Taxus cuspidata Cells in Suspension Culture

编号 zgly0000284111

文献类型 期刊论文

文献题名 Ce4+-Induced Apoptosis of Taxus cuspidata Cells in Suspension Culture

作者 葛志强  元英进  王艳东  马振毅  胡宗定 

作者单位 DepartmentofBiochemicalEngineering 

母体文献 Journal of Rare Earths;中国稀土学报: 英文版 

年卷期 2002,20(2)

页码 139-144

年份 2002 

分类号 Q942 

关键词 紫杉  细胞培养  悬浮培养  PCD  铈  稀土  细胞凋亡 

文摘内容 The standard detection hallmarks of apoptosis of Taxus cuspidata cells in suspension culture with Ce4+ were studied. The condensation and margination of chromatin were observed under the electron microscopy. DNA fragmentation ranged DNA ladder on agarose gel electrophoresis. TdT-mediated dUTP nick end labeling (TUNEL) analysis of the cells reveals that the nuclear DNA strand breaks can be identified by labeling free 3'-OH termini. These results suggest that Ce4+ can induce apoptosis of Taxus cuspidata cells and also indicate that there is a certain relationship between apoptosis and secondary metabolite preduct-Taxol。