Stable Transformation of Three Cultivars of Kentucky Bluegrass (Poa pratensis L.) by Particle Bombardment of Mature Seed—Derived Highly Regenerative Callus

编号 zgly0000310498

文献类型 期刊论文

文献题名 Stable Transformation of Three Cultivars of Kentucky Bluegrass (Poa pratensis L.) by Particle Bombardment of Mature Seed—Derived Highly Regenerative Callus

作者 CHAIBao-feng  LIANGAi-hua  KlauseKNielsen  GAOCai-xia  WANGWei  HUW 

作者单位 InstituteofHydrobiology  ChineseAcademyofSciences  Wuhan430072  P.R.China 

母体文献 Agricultural Sciences in China;中国农业科学: 英文版 

年卷期 2003,2(1)

页码 27-34,T001

年份 2003 

分类号 S543.9  S688.4 

关键词 肯塔基早熟禾  成熟种子  粒子照射  高再生愈伤组织  稳定转化  育种 

文摘内容 High embryogenic calli of three cutivars of Kentucky bluegrass,Md,Bd,and Gm, were induced from mature embryos,and were proliferated on medium K3 and K5. Embryogenic calli were transformed with plamids pDM803 and pBY520 by microprojectile bombardment. Fourty-two transgenic lines had been obtained. The highest efficiency of transformation reached to 3.7% for cv.Md,2.8% for cv.Gm, and 5% for cv. Bd. The micro nutriment of Cupric had significant effect on transformation. The emhryogenic callus cultured in dim-ligh condition had higher transformation efficiency than the green callus cultured in light condition for one month before transformation. The selective regime and selective pressure on the putaive transgenic plants were important for obtaining the desire number of transgenic plants. It also affected the copy number of integrated genes in the genomic DNA of transgenic plants。