Spatio-Temporal Expression Pattern of Six Novel Candidate Genes in Ginsenoside Biosynthesis from Panax ginseng C. A. Meyer

编号 zgly0000369442

文献类型 期刊论文

文献题名 Spatio-Temporal Expression Pattern of Six Novel Candidate Genes in Ginsenoside Biosynthesis from Panax ginseng C. A. Meyer

作者 Zhi-YongLUO  Shui-PingLIU  Xiang-HuiCHEN  YingRUAN  Jian-QingLUO  BinWEN  Chun-LinLIU  Wei-XinHU 

作者单位 InstituteofMolecularBiology  CropsGeneticEngineeringKeyLaboratoryofHunanProvince 

母体文献 Journal of Integrative Plant Biology;植物学报: 英文版 

年卷期 2005,47(5)

页码 622-631

年份 2005 

分类号 Q949.763.2  Q943.2 

关键词 人参  人参皂甙  生物合成  基因表达  萃取  候选基因  表达模式 

文摘内容 To explore the mode of the spatio-temporal expression of six newly discovered ginsenoside biosynthesis candidate gene transcripts, both Northern blotting and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) were used to elucidate the mRNA expression levels of the transcripts in various tissues and organs of Panax ginseng C. A. Meyer during different growth development stages. The six gene transcripts were all differentially expressed in cultured callus, root, stem, leaf, and seed.The mRNA expression levels were significantly higher in four-year-old roots than in one-year-old roots, and results of semi-quantitative RT-PCR assays were in accordance with those of Northern blotting analyses.The results strongly suggest that all six genes were differentially expressed at root-specific developmental stages. In particular, when a quiescent early stage culture suspension of P. ginseng cells was exposed to the ginsenoside biosynthesis-promoting elicitor Aspergillus niger polysaccharide, the GBR6 gene transcript response showed time-dependent increments and was parallel with ginsenoside productivity (P < 0.01).Overexpression of the GBR6 gene is likely to play a critically important role in the biosynthesis of ginsenosides.The results of the present study provided a background for the further elucidation of the structure and physiological function of these six candidate genes。