Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves

编号 040019004

推送时间 20190610

研究领域 森林培育 

年份 2019 

类型 期刊 

语种 英语

标题 Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves

来源期刊 planta

期 第190期

发表时间 20190504

关键词 Abiotic stress;  De novo transcriptome;  Drought stress;  Cellular stress response;  RNA-Seq;  Transcription;  factors;  Yerba mate; 

摘要 Ilex paraguariensis to overcome dehydration, we investigated (1) how gene expression varied between water-stressed and non-stressed plants and (2) in what way the modulation of gene expression was linked to physiological status and metabolite composition. A total of 4920 differentially expressed transcripts were obtained through RNA-Seq after water deprivation. Drought induced the expression of several transcripts involved in the ABA-signalling pathway. Stomatal closure and leaf osmotic adjustments were promoted to minimize water loss, and these responses were accompanied by a high transcriptional remodeling of stress perception, signalling and transcriptional regulation, the photoprotective and antioxidant systems, and other stress-responsive genes. Simultaneously, significant changes in metabolite contents were detected. Glutamine, phenylalanine, isomaltose, fucose, and malate levels were shown to be positively correlated with dehydration. Principal component analysis showed differences in the metabolic profiles of control and stressed leaves. These results provide a comprehensive overview of how I. paraguariensis responds to dehydration at transcriptional and metabolomic levels and provide further characterization of the molecular mechanisms associated with drought response in perennial subtropical species.

服务人员 孙小满

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