编号 040031703
推送时间 20211115
研究领域 森林培育
年份 2021
类型 期刊
语种 英语
标题 Decoding and recoding plant development
来源期刊 Plant Physiology
期 第317期
发表时间 20210826
关键词
plant species;
plant development;
synthetic
biology;
CRISPR/Cas9;
摘要 The development of multicellular organisms has been studied for centuries, yet many critical events and mechanisms of regulation remain challenging to observe directly. Early research focused on detailed observational and comparative studies. Molecular biology has generated insights into regulatory mechanisms, but only for a limited number of species. Now, synthetic biology is bringing these two approaches together, and by adding the possibility of sculpting novel morphologies, opening another path to understanding biology. Here, we review a variety of recently invented techniques that use CRISPR/Cas9 and phage integrases to trace the differentiation of cells over various timescales, as well as to decode the molecular states of cells in high spatiotemporal resolution. Most of these tools have been implemented in animals. The time is ripe for plant biologists to adopt and expand these approaches. Here, we describe how these tools could be used to monitor development in diverse plant species, as well as how they could guide efforts to recode programs of interest.
服务人员 孙小满
服务院士 尹伟伦
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