Engineering the plastid and mitochondrial genomes of flowering plants

编号 040036702

推送时间 20221031

研究领域 森林培育 

年份 2022 

类型 期刊 

语种 英语

标题 Engineering the plastid and mitochondrial genomes of flowering plants

来源期刊 Nature Plants

期 第367期

发表时间 20220829

关键词 flowering plants;  plastid genome;  mitochondrial genome;  CRISPR/Cas9;  Engineering;  transcription activator-like;  genome editing; 

摘要 Engineering the plastid genome based on homologous recombination is well developed in a few model species. Homologous recombination is also the rule in mitochondria, but transformation of the mitochondrial genome has not been realized in the absence of selective markers. The application of transcription activator-like (TAL) effector-based tools brought about a dramatic change because they can be deployed from nuclear genes and targeted to plastids or mitochondria by an N-terminal targeting sequence. Recognition of the target site in the organellar genomes is ensured by the modular assembly of TALE repeats. In this paper, I review the applications of TAL effector nucleases and TAL effector cytidine deaminases for gene deletion, base editing and mutagenesis in plastids and mitochondria. I also review emerging technologies such as post-transcriptional RNA modification to regulate gene expression, Agrobacterium- and nanoparticle-mediated organellar genome transformation, and self-replicating organellar vectors as production platforms.

服务人员 孙小满

服务院士 尹伟伦

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