楸子初代组织培养中防止外植体褐化的研究

编号 lyqk006230

中文标题 楸子初代组织培养中防止外植体褐化的研究

作者 盖琼辉 

作者单位 陇东学院,甘肃 庆阳 745000

期刊名称 林业科技通讯 

年份 2017 

卷号 0

期号 4

栏目名称 种苗繁育 

中文摘要 为了防止楸子(Malus prunifolia Willd.Borkh)初代组织培养外植体褐化,设置选用不同外植体、消毒方法、培养温度、光照、6-BA浓度及不同防褐化剂类型与浓度研究楸子离体培养过程中外植体褐化影响因素。结果表明,选用当年生新枝上1~3cm的带芽茎段作为材料,使用0.1% HgCl₂消毒,4℃低温暗培养5d,并在培养基中添加1~2g/L抗坏血酸或0.5~2g/L聚乙烯吡咯烷酮及0.5mg/L的6-BA可以有效防止褐化,且植株生长旺盛,色泽正常,生长势较好。

关键词 楸子  Malus prunifolia Willd.Borkh  初代培养  外植体  防止褐化 

英文标题 Study on Explants of Malus prunifolia Prevented from Browning in Early Tissue Culture

英文摘要 In order to prevent explant from browning in early generation culture of Malus prunifolia Willd.Borkh, the effects of different kinds of explants, disinfection methods, culture temperature, illumination, concentrations of 6-BA and browning agent on explant browning tissue culture were selected and studied.Results show that 1~3cm as explant material containing buds of stem section can be prevent from browning better, by using 0.1% HgCl₂ to disinfect, culturing under 4℃ for 5days.The concentration of 1~2g/L of Vc or 0.5~2g/L of PVP supplemented to medium can be inhibited explant from browning;The concentration of 0.5g/L of 6-BA supplemented to medium can reduce explant browning and result in a good growth.

英文关键词 Malus prunifolia Willd.Borkh;early generation culture;explant;prevent from browning

起始页码 30

截止页码 33

分类号 S723.1+32.6

DOI 10.13456/j.cnki.lykt.2017.04.008

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