云南甜龙竹组培及组培苗移栽技术研究

编号 lyqk007507

中文标题 云南甜龙竹组培及组培苗移栽技术研究

作者 钟永莉  陈玟旭  谭宏超 

作者单位 云南师范大学生命科学学院 昆明 650500

期刊名称 世界竹藤通讯 

年份 2019 

卷号 17

期号 2

栏目名称 学术园地 

中文摘要 选择6种类型的云南甜龙竹外植体，经不同浓度的升汞溶液消毒一定时间，在添加不同浓度6-BA的MS培养基中进行组培试验，以筛选云南甜龙竹组培繁殖的适宜条件；同时，以1年生云南甜龙竹组培苗为材料，在6种育苗基质和4种育苗环境下试验组培苗的生长情况，以筛选适宜组培苗生长的最佳条件。结果表明：云南甜龙竹组培繁育以1年生种子苗茎段作为外植体，用0.10%~0.15%的升汞消毒6~9 min，使用标准MS培养基，添加浓度为1.5~2.0 g/mL的6-BA，能够获得较高的芽诱导率；1年生组培苗移栽时，采用大棚套小棚的移栽环境，以植物碎沫作为栽培基质，苗木成活率较高，幼苗生长较好。

关键词 云南甜龙竹  组织培养  外植体  芽诱导率  组培苗  移栽环境 

基金项目 云南珍竹农业科技有限公司与马来西亚竹子绿世界有限公司资助项目。

英文标题 A Study of Tissue Culture and Seedling Transplanting Technology for Dendrocalamus brandiss

作者英文名 Zhong Yongli, Chen Wenxu, Tan Hongchao

单位英文名 College of Life Sciences, Yunnan Normal University, Kunming 650500, China

英文摘要 Six types of explants were selected from Dendrocalamus brandiss and after disinfected with different concentrations of mercuric chloride solution for a certain period of time, they were cultivated in MS medium supplemented with different concentrations of 6-BA, in order to screen the appropriate conditions for tissue culture of D. brandiss. Meanwhile, 1-year-old tissue culture seedlings were tested under 6 kinds of seedling substrates and 4 different environmental conditions to screen the optimal conditions suitable for the growth of tissue culture seedlings. The results showed that the explants of young culm from 1-year-old seedlings which were disinfected with 0.10%-0.15% mercuric chloride for 6-9 min and cultivated in standard MS medium with 1.5-2.0 g/mL of 6-BA, could achieve higher bud induction rate. When the 1-year-old tissue culture seedlings were transplanted, the seedlings were covered in greenhouse and cultivated with the fine particles made from plant debris, which could achieved higher survival rate and better growth.

英文关键词 Dendrocalamus brandiss;tissue culture;explant;bud induction rate;tissue culture seedling;transplanting condition

起始页码 31

截止页码 35

作者简介 钟永莉(1995-),女,在校本科生,从事竹子组织培养研究。E-mail:2727141387@qq.com

通讯作者介绍 谭宏超(1963-),男,教授,从事竹产业教学研究开发工作。E-mail:ynbamboo@126.com

E-mail ynbamboo@126.com

DOI 10.13640/j.cnki.wbr.2019.02.007

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