不同浓度氮处理毛竹水通道蛋白基因表达模式及其调控网络

编号 lyqk010049

中文标题 不同浓度氮处理毛竹水通道蛋白基因表达模式及其调控网络

作者 朱成磊  林泽铭  李天阔  高志民 

作者单位 国际竹藤中心竹藤资源基因科学与基因产业化研究所, 国家林业和草原局/北京竹藤科学与技术重点开放实验室 北京 100102

期刊名称 世界竹藤通讯 

年份 2022 

卷号 20

期号 6

栏目名称 学术园地 

中文摘要 氮和水分平衡对植物生长发育具有重要作用，在长期进化过程中植物体内形成了氮运输和水分平衡的复杂调控网络。为揭示竹子速生的内在调控机制，研究了不同浓度氮处理下毛竹根中水通道蛋白(AQP)基因的表达模式，并预测了其表达调控网络。结果表明，在不同浓度氮处理下，在毛竹根的转录本中共鉴定到30个差异表达的PeAQPs，包括14个PePIPs、8个PeTIPs、7个PeNIPs和PeSIP2-1；随着氮浓度的提高，PeAQPs的表达模式主要分为2类：一类呈上调表达，另一类呈下调表达。在这些差异表达AQP基因的启动子中鉴定到了许多转录因子(TF)的结合元件，同时通过筛选差异表达TFs，构建了差异表达TFs和PeAQPs的共表达网络。代表性差异表达TFs和PeAQPs表达的相关性分析和共表达结果表明，这些TF可能参与调控毛竹中水分和氮的协调运输。研究结果可为深入解析毛竹快速生长的分子机制提供参考。

关键词 毛竹  氮处理  水通道蛋白  基因表达模式  共表达网络 

基金项目 国家自然科学基金项目(31971736)。

英文标题 Expression Pattern of Aquaporin Genes in Moso Bamboo under Different Nitrogen Concentrations and Its Regulating Network

作者英文名 Zhu Chenglei, Lin Zeming, Li Tiankuo, Gao Zhimin

单位英文名 Institute of Gene Science and Industrialization for Bamboo and Rattan Resources, International Center for Bamboo and Rattan;Key Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo and Rattan Science and Technology, Beijing, 100102, China

英文摘要 Nitrogen and water balance play important roles in the growth and development of plants, and a complex regulatory network of nitrogen transport and water balance has been formed in plants during their long-term evolution. The paper studies the expression patterns of aquaporin (AQP) gene in the roots of nitrogen-treated moso bamboo (Phyllostachys edulis) and predicts its expression regulation network, to reveal the internal regulation mechanism of bamboo rapid growth. The results show that a total of 30 differentially expressed PeAQPs, including 14 PePIPs, 8 PeTIPs, 7 PeNIPs and PeSIP2-1, are identified in the transcripts of moso bamboo roots under different nitrogen concentrations. The expression patterns of PeAQPs are mainly divided into two types, i.e, up-regulated and down-regulated, with the increase of nitrogen concentration. A number of transcription factor (TF) binding elements are identified in the promoters of these differentially expressed PeAQPs, and a co-expression network of TFs and PeAQPs is constructed by screening for the differentially expressed TFs. The correlation analysis and co-expression results of representative differentially expressed TFs and PeAQPs indicate that these TFs could be involved in regulating the coordinated transport of water and nitrogen in moso bamboo. The results would provide a reference for further understanding the molecular mechanism of rapid growth of moso bamboo.

英文关键词 moso bamboo;nitrogen treatment;aquaporin;gene expression pattern;co-expression network

起始页码 21

截止页码 29

作者简介 朱成磊,博士研究生,研究方向为竹子速生遗传调控,E-mail:zhuchenglei@icbr.ac.cn

通讯作者介绍 高志民,研究员,博士生导师,研究方向为竹藤生长发育的分子基础,E-mail:gaozhimin@icbr.ac.cn

E-mail gaozhimin@icbr.ac.cn

DOI 10.12168/sjzttx.2022.06.004

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