分子标记技术在桉树育种研究中的应用进展

编号 lyqk003894

中文标题 分子标记技术在桉树育种研究中的应用进展

作者 刘果  谢耀坚 

作者单位 国家林业局桉树研究开发中心,广东湛江 524022;国家林业局桉树研究开发中心,广东湛江 524022

期刊名称 世界林业研究 

年份 2012 

卷号 25

期号 3

栏目编号 1

栏目名称 专题论述 

中文摘要 桉树是全球重要的造林树种,也是林木遗传研究的模式树种,分子标记技术在桉树遗传育种中的研究和应用具有重要的理论意义和实用价值。文中概述了几种主要的分子标记技术及其在桉树遗传育种中的应用,并提出分子标记技术在桉树遗传育种中的应用前景和研究展望。

关键词 分子标记技术  桉树  遗传育种  RFLP  RAPD  AFLP  SSR  ISSR  SNP 

基金项目 桉树生态经营及产业升级关键技术研究(201104003)

英文标题 Application of Molecular Marking Technologies in Heredity Breeding of Eucalyptus

作者英文名 Liu Guo and Xie Yaojian

单位英文名 China Eucalypt Research Center,State Forestry Administration,Zhanjiang 524022,Guangdong,China;China Eucalypt Research Center,State Forestry Administration,Zhanjiang 524022,Guangdong,China

英文摘要 Eucalyptus is an important tree species on the global scale, and also it is the model tree in forest genetic research. Therefore, the research and application of molecular marking technology in heredity breeding of Eucalyptus have important theoretical significance and practical value. This paper described several major molecular marker technologies and their application to heredity breeding of Eucalyptus, and prospected the future application and study of these technologies.

英文关键词 molecular marking technology;Eucalyptus;genetic breeding;RFLP;RAPD;AFLP;SSR;ISSR;SNP

起始页码 19

截止页码 25

投稿时间 2011/10/10

分类号 S718.46,S722.3

参考文献 [1] 周延清.遗传标记的发展[J].生物学通报,2000,35(5):17-18.
[2] 郭勇.利用EST-CAPS标记构建桉树遗传图谱.北京:中国林业科学研究院,2008.
[3] 彭学贤.植物分子生物技术应用手册[M].北京:化学工业出版社,2006:98-100.
[4] Thamarus K A,Groom K,Murrell J,et al.A genetic linkage map for Eucalyptus globulus with candidate loci for wood,fibre and floral traits[J].Theoritical and Applied Genetics,2002,104(2/3):379-387.
[5] Agrama H A,George T L,Salah S F.Construction of genome map for Eucalyptus camaldulensis Dehn[J].Silvae Genetica,2002,51(5/6):201-206.
[6] Elliott C,Byrne M.Genetic diversity within and between natural populations of Eucalyptus occidentalis(Myrtaceae) [J].Silvae Genetica,2003,52(3/4):169-173.
[7] Elliott C P,Byrne M.Phylogenetics and the conservation of rare taxa in the Eucalyptus angustissima complex in Western Australia[J]. Conservation Genetics,2004,5(1):39-47.
[8] 胡欲清,赵树进.RAPD技术及其在植物研究中的应用[J].生物技术通报,2010(5):74-77.
[9] 刘晓宇.RAPD分子标记技术概述及应用[J].科技创新与生产力,2010(9):98-99.
[10] Skabo S J,Vaillancourt R E,Potts B M.Fine-scale genetic structure of Eucalyptus ssp.globulus forest revealed by RAPDs[J].Australian Joumal of Botany,1998,46(6):583-594.
[11] Nesbitt K A,Potts B M,Vaillancourt R E,et al.Fingerprinting and pedigree analysis in Eucalyptus globulus using RAPDs[J].Silvae Genetica,1997,46(1):6-11.
[12] Verhaegen D,Plomion C.Genetic mapping in Eucalyptus urophylla and Eucalyptus grandis using RAPD markers[J].Genome,1996,39(6):1051-1061.
[13] Grattapaglia D,Bertolucci F L G,Penchel R,et al.Genetic mapping of quantitative trait loci controlling growth and wood quality traits in Eucalyptus grandis using a maternal half-sib family and RAPD markers[J].Genetics,1996,144(3):1205-1214.
[14] 甘四明,施季森,白嘉雨,等.尾叶桉和细叶桉无性系的RAPD指纹图谱构建[J].南京林业大学学报,1999,23(1):11-14.
[15] 甘四明,施季森,白嘉雨,等.RAPD标记在桉属种间杂交一代的分离方式研究[J].林业科学研究,2001,14(2):125-130.
[16] 甘四明,白嘉雨,吴坤明,等.7个桉树杂交亲本RAPD位点多态性和杂合性研究[J].林业科学研究,2003,39(2):162-167.
[17] 潘天玲,刘友全,李坤平.RAPD标记在韦塔桉种源遗传结构上的应用[J].生命科学研究,2003,7(1):89-94.
[18] 李志辉,杨模华.巨桉种源遗传多样性的RAPD分析[J].中南林学院学报,2003,23(4):5-9.
[19] 李发根,甘四明,李梅,等.利用RAPD标记进行桉树亲本遗传变异的研究[J].广东林业科技,2005,21(3):1-5.
[20] 刘静.AFLP分子标记的发展及应用[J].山东农业科学,2010(5):10-14.
[21] Margues C M,Araujo J A,Ferreira J G,et al.AFLP genetic maps of Eucalyptus globulus and E.tereticornis[J].TAG Theoritical and Applied Genetics,1998,96(6/7):727-737.
[22] Myburg A A,Griffin A R,Sederoff R R,et al.Comparative genetic linkage maps of Eucalyptus grandis,Eucalyptus globulus and their F₁ hybrid based on a double pseudobackcross mapping approach[J]. TAG Theoritical and Applied Genetics,2003,107(6):1028-1042.
[23] Alexander A Myburg,Claus Vogl,A Rod Griffin,et al.Genetics of postzygotic isolation in Eucalyptus:whole-genome analysis of barriers to introgression in a wide interspecific cross of Eucalyptus grandis and E.globulus[J].Genetics,2004,166(3):1405-1418.
[24] Mo X Y,Long T,Liu Z,et al.AFLP analysis of somaclonal variations in Eucalyptus globulus[J].Biologia Plantarum,2009,53(4):741-744.
[25] Hamada H,Petrino M G,Kakunaga T.A novel repeated element with Z-DNA-forming potential is widely found in evolutionarily diverse eukaryotic genomes[J].Proceeding of the National Academy of Sciences of the United States America,1982,79(21):6465-6469.
[26] Marques C,Brondani R,Grattapaglia D,et al.Conservation and synteny of SSR loci and QTLs for vegetative propagation in four Eucalyptus species[J].TAG Theoritical and Applied Genetics,2002,105(2/3):474-478.
[27] Brondani R P V,Brondani C,Tarchini R,et al.Development,characrterization and mapping of microsatellite markers in Eucalyptus grandis and E.urophylla[J].TAG Theoritical and Applied Genetics,1998,97(5/6):816-827.
[28] Brondani R P,Brondani C,Grattapaglia D.Towards a genus-wide reference linkage map for Eucalyptus based exclusively on highly informative microsatellite markers[J].Molecular Genetic Genomics,2002,267(3):338-347.
[29] 何旭东.桉树杂种优势及其分子标记辅助选择研究.南京:南京林业大学,2010.
[30] 李发根.尾叶桉和细叶桉STS标记连锁图谱构建及生长状况QTL定位研究.北京:中国林业科学研究院,2010.
[31] Van Der Nest M A,Steenkamp E T,Wingfield B D,et al. Development of simple sequence repeat(SSR) markers in Eucalyptus from amplified inter-simple sequence repeats(ISSR)[J].Plant Breeding,2000,119(5):433-436.
[32] 陈晓明,王以红,蔡玲,等.几个桉树优良无性系的ISSR指纹图谱分析[J].西部林业科学,2009,38(2):57-61.
[33] 田华.桉树4个种遗传多样性的ISSR分析及STS标记开发初探.长沙:中南林业科技大学,2009.
[34] 张党权,田华,谢耀坚,等.细叶桉遗传多样性的ISSR分析[J].中南林业科技大学学报,2009,29(5):91-94.
[35] 曾艳玲,谭晓风,张党权,等.基于ISSR分子标记的桉树遗传分析[J].种子,2010,29(1):24-26.
[36] 王强,李卫真,张永云.SNPs技术的研究进展及应用[J].四川畜牧兽医,2010(8):31-33.
[37] 李兆波,吴禹,王岩,等.SNP标记技术及其在农作物育种中的应用[J].辽宁农业职业技术学院学报,2010,5(3):8-9.
[38] Bala R Thumma,Maureen F Nolan,Robert Evans,et al. Polymorphisms in cinnamoyl CoA reductase(CCR)are associated with variation in microfibril angle in Eucalyptus spp.[J].Genetics,2005,171(3):1257-1265.
[39] Carsten Külheim,Suat Hui Yeoh,Jens Maintz,et al.Comparative SNP diversity among four Eucalyptus species for genes from secondary metabolite biosynthetic pathways[J].BMC Genomics,2009,10(1):452.
[40] 张晓红.桉树EST-SNP的开发及EST图谱构建.南京:南京林业大学,2009.
[41] 刘振盼.分子生物学技术在林木遗传育种研究中的应用[J].农业科技与装备,2009(5):23-25.
[42] 郭勇.利用EST-CAPS标记构建桉树遗传图谱.北京:中国林业科学研究院,2008.
[43] 刘海龙,王以红,陈博雯,等.利用ISSR标记方法鉴定5个桉树优良无性系[J].西部林业科学,2011(3):66-68.
[44] 刘海龙,王以红,吴幼媚,等.桉树无性系与亲本及桉属其它种遗传关系分析[J].广西林业科学,2011(1):4-7.
[45] 陈晓明,王以红,蔡玲,等.几个桉树优良无性系的ISSR指纹图谱分析[J].西部林业科学,2009(2):57-61.
[46] 张照远.桉树EST-CAPS标记的开发及其用于遗传图谱构建的研究.福州:福建农林大学,2006.

PDF全文 浏览全文