栓皮栎体胚诱导关键影响因素研究

编号 zgly0000363489

文献类型 期刊论文

文献题名 栓皮栎体胚诱导关键影响因素研究

学科分类 220.99;林学其他学科

作者 张存旭  姚增玉  赵忠 

作者单位 西北农林科技大学 

母体文献 林业科学 

年卷期 2005,41(2)

页码 174-177,i004

年份 2005 

分类号 S791.180.5  TP311.13 

关键词 体胚诱导  栓皮栎  with  6-BA  impact  was  light  great  and  the  more  high  High  best  part  The  in  to  can  MS  WPM  big  med  cal  for  mg 

文摘内容 Immature zygotic embryos of Quercus variabilis were as explants to induce somatic embryogenesis. Several factors influencing somatic embryogenesis have been assayed. Somatic embryos can be induced in MS and WPM basal medium, but there was more quantity, big size and high induction rate in MS medium. Induction rate was not significant cultured in light and dark condition. Zygotic embryos, collected in middle of July, gave higher rate of somatic embryogenesis than those collected on the earlier or later date. By adding 6-BA in medium individually, somatic embryogenesis appeared directly on the zygotic embryos without detectable callus. Secondary embryogenesis appeared in medium with 2,4-D individual or combined with 6-BA or TDZ. High induction frequency of 90% was achieved in MS medium supplemented with O. 5 mg·L^-1 6-BA and 2,4-D, whereas the rate in hormone-free medium was only 16.7%. The genotypes of mother trees had an great impact on the inducing rate. Zygotic embryo surgery treatments were not favorable to embryogenesis. It was best to inoculate with entire zygotic embryos. The hypocotyl was a crucial part on somatic embryogenesis for Q. variabilis。