Multiplication of Elaeagnus angustifolia L. from a Single Shoot in Vitro

编号 zgly0000338919

文献类型 期刊论文

文献题名 Multiplication of Elaeagnus angustifolia L. from a Single Shoot in Vitro

学科分类 220.99;林学其他学科

作者 LIYanju  YANGZhengli  SHIGERUHisajima 

作者单位 InstituteofAgriculturalResourceandDivisionPlanning  ChineseAcademyofAgriculturalSciencesZhongguancunSouthSt..No12  Beijing100081  P.R.China  InstituteofAppliedBiochemistry  UniversityofTsukuba  Ibaraki305Japan 

母体文献 中国林业科技: 英文版 

年卷期 2004,3(1)

页码 93-98

年份 2004 

分类号 S792.99 

关键词 嫩芽  组织培养  培养基  激素  吲哚-3-丁酸  激动素  胡颓子 

文摘内容 Multiplication of Elaeagnus angustifolia L. was examined in vitro successively from a single shoot under the specified condition of different media, plant growth regulators, pH value and sucrose concentration. It was showed that MMS1 was the most suitable medium on shoot multiplication among 5 media concerned;BAP was the most effective one among all the cytokinin involved, BAP, KN, TDZ and ZT; the explunt of the top half-part from a shoot produced more new shoots than that of the foot half-part did; more new shoots (>2 cm) were produced under 3 % sucrose between the concentrations with top half-part explants; shoots could grow well between pH 4.4 and pH 7.0, and the biggest number of shoots was produced in pH 5.6, while in pH 5.8 the maximum rooting rate appeared. As a result, the combination of 0.5μM BAP and 0.1μM IBA on MMS1 medium induced the maximum shoot multiplication. The number of shoot amplified 3 times in 1 month, and 3^12 shoots (>2 cm) might be theoretically multiplied annually from a single shoot。