大腹园蛛Avg1 cDNA的克隆和序列分析

编号 zgly0000356765

文献类型 期刊论文

文献题名 大腹园蛛Avg1 cDNA的克隆和序列分析

作者 任洪林  柳增善  卢士英  潘风光 

作者单位 解放军军需大学军事兽医系  东北林业大学野生动植物资源学院 

母体文献 中国生物化学与分子生物学报 

年卷期 2004,20(4)

页码 561-564

年份 2004 

分类号 Q78 

关键词 Araneus  ventricousus  major  ampullate  gland  cathepsin  B  gene  cloning  sequence  analysis 

文摘内容 Quantity and activity of cathepsin B (CB) are increased during the progress of carcinoma cells' invasion and metastasis. So, CB is being applied to the diagnosis of cancers and inhibiting their diffusion. The complete Avgl eDNA was randomly cloned from the cDNA library of major ampullate gland of Araneus ventricousus. It is 1 253 bp and encode 334 amino acids in its 1 002 bp encoding region. The molecular weight of the protein is 36 953.00.GenBank accession number is AY302573. The 3′ non-coding region is composed of 179 bp with a polyadenylation signal AATAAA sequence appearing at the position 129 nt and the poly(A) tail is at the position 153 nt downstream of stop codon TAA. The signal peptide cleavage site of its deduced protein is between codon 16 and 17. Two glycosylation sites of AsnThrThr and AsnValSer, respectively, appear at codon 23 and 202. The high homology genes are not found in all genes known in NCBI, but the typical conserved domain of peptidase-C1 has been detected in NCBI BLASTp, and high homologies with CB of some kinds of creatures are shown. The objective function of the Avgl has not been studied in the spide silk gland yet。