油茶苗圃炭疽病菌抗药性研究(英文)

编号 zgly0001433510

文献类型 期刊论文

文献题名 油茶苗圃炭疽病菌抗药性研究(英文)

作者 李河  周国英  章怀云  刘君昂  彭宽 

作者单位 中南林业科技大学林业生物技术湖南省重点实验室  中南林业科技大学生物技术中心实验室林学院 

母体文献 植物病理学报 

年卷期 2012年02期

年份 2012 

分类号 S763.7 

关键词 Camellia oleifera Abel.  Colletotrichum gloeosporioides Penz  β-tubulin gene  Carbendazim-resistance  Single base-pair point mutation 

文摘内容 The fungicide resistance of anthracnose in Camellia oleifera nurseries was investigated in Liuyang,Changning,and other regions in Hunan Province.The Colletotrichum gloeosporioides strains from the former two regions were highly resistant to carbendazim.After subcultured for 10 generations on fungicide-free medium,the resistant strains grew well on the medium containing carbendazim 450 μg/mL and suggesting that its resistance was stable.The β-tubulin genes from the resistant and susceptible strains were cloned and sequenced.The coding region was 1 344 bp nucleotides and predicted to encode a protein with 447 amino acids.Comparison of the β-tubulin amino acid sequences between resistant and susceptible strains of Colletotrichum gloeosporioides revealed that a mutation leading to an amino acid substitution at the position 198 from glutamic acid in the susceptible strain to alanine in the resistant strain.Finally,the main morphological characteristics of C.gloeosporioides were descripted,but it could not be used to determine the resistance to carbendazim.