Optimization of the uidA Gene Transfer of Rosa hybrida via Agrobacterium tumefaciens: an Assessment of Factors Influencing the Efficiency of Gene Transfer

编号 zgly0000345087

文献类型 期刊论文

文献题名 Optimization of the uidA Gene Transfer of Rosa hybrida via Agrobacterium tumefaciens: an Assessment of Factors Influencing the Efficiency of Gene Transfer

作者 GaoLiping  BaoManzhu 

作者单位 KeyLaboratoryofHorticultureBiology 

母体文献 Forestry Studies in China;中国林学: 英文版 

年卷期 2004,6(3)

页码 9-14

年份 2004 

分类号 Q949.751.8  Q943.2 

关键词 蔷薇属  种间杂交  基因  土壤杆菌  瞬间表达 

文摘内容 To develop a transformation protocol of Rosa hybrida ‘Samantha' via Agrobacterium tumefaciens, the authors examined the effect of different factors on T-DNA transfer by measuring transient expression levels of an intron-containing β-glucuronidase gene. The results indicate that explant, light condition, salt concentration and acetosyringone (AS) concentration in co-culture medium are the most important factors, and factors like co-culture temperature, co-culture period and bacteria density have a strong effect on the growth of bacteria and then T-DNA transfer. Optimized co-cultivation was performed by inoculation of embryogenic callus with bacteria at a density of OD600=0.5-0.8 for 20 min and co-culture in darkness under 23 ℃ on medium with 1/2 MS salts and 300 μmol-L 1 AS for 3 d。