基于Ypt1基因为靶标的木香疫病病组织及病田土壤分子检测技术(英文)

编号 zgly0001459689

文献类型 期刊论文

文献题名 基于Ypt1基因为靶标的木香疫病病组织及病田土壤分子检测技术(英文)

作者 孟军  刘燕  方志翔 

作者单位 环境保护部南京环境科学研究所国家环境保护生物安全重点实验室 

母体文献 植物病理学报 

年卷期 2013年01期

年份 2013 

分类号 S432.4 

关键词 molecular detection  Ypt1 gene  oomycetes 

文摘内容 A species-specific PCR assay was established for rapid and accurate detection of the oomycete pathogen Phytophthora tentaculata in diseased plant tissues and infected soil.A pair of species-specific primers Pt1/Pt2 were designed on the basis of Ras-related protein(Ypt1) gene sequences of the Phytophthora species.PCR amplification with the Pt primers resulted in a 386 bp product only from isolates of P.tentaculata.The detection threshold with Pt primers was 100 pg of genomic DNA.A nested PCR procedure was developed using Ypt1F/Ypt1R as the first-round amplification primers and Pt1/Pt2 as the second-round primers,which increased the detection sensitivity 100-fold to 1 pg.PCR using these Pt primers can also be used to detect P.tentaculata in naturally infected plant tissues and soil.The PCR-based method developed in this study provides a rapid and sensitive tool for detection of P.tentaculata.