编号
zgly0000325379
文献类型
期刊论文
文献题名
二次回归正交设计在微型月季组织培养中的应用
学科分类
220.5010;园林植物学
作者单位
北京林业大学生物科学与技术学院
母体文献
林业科学
年卷期
2004,40(4)
页码
189-192
年份
2004
分类号
S685.12
关键词
微型月季
组织培养
二次回归正交设计
激素
6-BA
NAA
培养基
文摘内容
Quadratic regressive factorial experiment was used to optimize concentrations of 6-BA and NAA added in MS midum for shoot multiplication of miniature rose ( Rosa cv. Sonnen Kind). The most amount of shoots was achieved by combining 6-BA 1.93 mg·L^-1, NAA 0.33mg·L^-1 into MS midum. The 6-BA and NAA concentrations were in a range of 1.68~2.75mg·L^-1 and 0.07~0.54mg·L^-1, respectively, and each 10 explants (stems with one auxiliary bud) cultured on the midum for 40 days, then 50 shoots occurred. The probability of the event was 95%。