Successful Agrobacterium-mediated transformation of Populus tomentosa with apple SPDS gene

编号 zgly0001334998

文献类型 期刊论文

文献题名 Successful Agrobacterium-mediated transformation of Populus tomentosa with apple SPDS gene

母体文献 Forestry Studies in China 

年卷期 2008年03期

年份 2008 

分类号 S792.11 

关键词 salt tolerance  spermidine synthase gene  transformation  Populus tomentosa 

文摘内容 The problem of salinized soils has become one of the most serious constraints to agricultural and forest productivity. With the purpose of enhancing salt stress tolerance of Populus tomentosa, we transformed this tree species with spermidine synthase (SPDS) genes derived from an apple by an Agrobacterium-mediated method. Four transgenic clones were confirmed by PCR and Southern blot analysis. As well, the expression of introduced SPDS genes was analyzed by real-time quantitative PCR.