Programmed cell death during terminal bud senescence in a sympodial branching tree, Eucommia ulmoides

编号 zgly0000341427

文献类型 期刊论文

文献题名 Programmed cell death during terminal bud senescence in a sympodial branching tree, Eucommia ulmoides

学科分类 220.99;林学其他学科

作者 XUWenjie  Kalima-N＇Koma  MWANGE  CUIKeming 

作者单位 CollegeofLifeSciences  PekingUniversity  Beijing100871  China 

母体文献 Progress in Natural Science;自然科学进展: 英文版 

年卷期 2004,14(8)

页码 694-699

年份 2004 

分类号 S794.1  Q945.48 

关键词 杜仲橡胶  DNA碎裂  程序细胞凋亡  终端芽老化  合轴分支树种  DNaSe  原位标签 

文摘内容 Eucommia ulmoides Oliv. is a typical sympodial branching tree. The apical bud of the branch ages and dies every year,replaced by the nearby axillary bud in the second year. Structural assays and a series of biochemical analyses were performed to analyze the senescence mechanism in the apical bud. It was revealed that most cells of the apical bud underwent the programmed cell death (PCD) during the senescence: the chromosomes were congregated and the nuclear contents were condensed, as shown by 4', 6-diamidino-2-phenylindole (DAPI) fluorescence. DNA fragmentation was detected during senescence using the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end in situ labeling (TUNEL) method, coincident with the appearance of a DNA ladder. Moreover, a20 kD DNase related to fragmentation was found. PCD was initiated first in the young leaves, leaf primordia and peripheral zone cells,then in the central mother cells and initial layer cells in the apical meristem. The terminal buds remain in vegetative growth during senescence, in contrast to buds of many annual plants。