Direct Adventitious Bud Induction and Plant Regeneration of Rosa hybrida Samantha

编号 zgly0000360275

文献类型 期刊论文

文献题名 Direct Adventitious Bud Induction and Plant Regeneration of Rosa hybrida Samantha

学科分类 220.5010;园林植物学

作者 GAOLi-ping  BAOMan-zhu 

作者单位 KeyLaboratoryofHorticulturalPlantBiology  CollegeofHorticultureandForestrySciences  MinistryofEducation/HuazhongAgriculturalUniversity  Wuhan430070  P.R.China 

母体文献 Agricultural Sciences in China;中国农业科学: 英文版 

年卷期 2005,4(2)

页码 101-105

年份 2005 

分类号 S685.12 

关键词 玫瑰  种间杂交  不定芽  组织培养  植株再生 

文摘内容 Effect of explant, site of leaflet, induction period in the dark and combinations of plant growth regulators on direct adventitious bud induction and plant regeneration of Rosa hybrida Samantha was investigated. The results showed that after an induction period of 8 d on MS medium with 1.5 mg L4 TDZ and 0.05 mg L-1 NAA in the dark and a subculture onMS medium with 0.5 mg L-1 BA and 0.01 mg L-1 NAA under light, the best plant regeneration was obtained and theregeneration frequencies of leaflets and petioles were 51.8 and 10% respectively. There was no significant difference in regeneration ability between leaflets at different sites of the compound leaves, longer time of induction in the dark or high concentration of auxin would cause callus formation, which was disadvantageous for shoot regeneration, and the regeneration frequency was significantly reduced. This regeneration system could be applied for genetic transformation of this cultivar in the future。