Plants regenerated from mesophyll protoplasts of white mulberry

编号 zgly0000109966

文献类型 期刊论文

文献题名 Plants regenerated from mesophyll protoplasts of white mulberry

学科分类 220.1520;林木遗传学

作者 WEIZHIMING  ZHIHONGXU 

作者单位 NationallaboratoryofPlantMolecularGenetics 

母体文献 Cell Research;细胞研究: 英文版 

年卷期 1994,4(2)

页码 183-189

年份 1994 

分类号 Q943.1  S888.32 

关键词 白桑  叶肉原生质体  植株再生 

文摘内容 Morus alba(white mulberry) mesophyll protoplasts were isolated from leaves of 30-45 day old sterile shoots,with protoplast yields of 2.5×10^7 g^-1/F.W.after purification.The protoplasts were cultured in a modified K8P liquid medium containing 0.2mg/L 2,4-(2,4-Dichlorophenoxy acetic acid),1mg/L NAA(Naphthyl acetic acid) and 0.5mg/L BA(6-benzylaminopurine).A low plating density(5×10^4/ml) proved to be favourable to the division of protoplast-derived cells.The first division occurred 4 days after culture,and the division frequency reached 24% at 10 days.A number of cell colonies and microcalli formed in 6 weeks.The microcalli were transferred onto MSB medium with 0.5mg/L NAA and 0.5mg/L BA for further proliferation.Shoot formation was initiated when the calli of 3-4 mm in size were transferred onto MSB differentiation medium with 0.1mg/L NAA and 1mg/L BA.The frequency of shoot formation was 35%.The shoots of 4-5 cm in height were excised from the callus and rooted on half strength MS medium with 0.5 mg/L IBA and 0.1 mg/L BA.After transplantation into pots,the regenerated plants grew vigorously in the phytotron。