Cotton AnnGh3 Encoding an Annexin Protein is Preferentially Expressed in Fibers and Promotes Initiation and Elongation of Leaf Trichomes in Transgenic Arabidopsis

编号 zgly0001461303

文献类型 期刊论文

文献题名 Cotton AnnGh3 Encoding an Annexin Protein is Preferentially Expressed in Fibers and Promotes Initiation and Elongation of Leaf Trichomes in Transgenic Arabidopsis

作者 Bing Li  Deng-Di Li  Jie Zhang  Hui Xia  Xiu-Lan Wang  Ying Li  Xue-Bao Li 

作者单位 Hubei Key Laboratory of Genetic Regulation and Integrative Biology  College of Life Sciences  Central China Normal University 

母体文献 Journal of Integrative Plant Biology 

年卷期 2013年10期

年份 2013 

分类号 S562 

关键词 Annexin  cotton(Gossypium hirsutum)  fiber development  gene expression 

文摘内容 The annexins are a multifamily of calcium‐regulated phospholipid‐binding proteins.To investigate the roles of annexins in fiber development,four genes encoding putative annexin proteins were isolated from cotton (Gossypium hirsutum) and designated AnnGh3,AnnGh4,AnnGh5,and AnnGh6.Quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) results indicated that AnnGh3,AnnGh4,and AnnGh5 were preferentially expressed in fibers,while the transcripts of AnnGh6 were predominantly accumulated in roots.During fiber development,the transcripts of AnnGh3/4/5 genes were mainly accumulated in rapidly elongating fiber s.With fiber cells further developed,their expression activity was dramatically declined to a relatively low level.In situ hybridization results indicated that AnnGh3 and AnnGh5 were expressed in initiating fiber cells (0–2 DPA).Additionally,their expression in fiber s was also regulated by phytohormones and [Ca2t].Subcellular localization analysis discovered that AnnGh3 protein was localized in the cytoplasm.Overexpression of AnnGh3 in Arabidopsis resulted in a signifcant increase in trichome density and length on leaves of the transgenic plants,suggesting that AnnGh3 may be involved in fiber cell initiation and elongation of cotton.