大叶黄杨无芽茎段不定芽再生及其起源

编号 zgly0000361727

文献类型 期刊论文

文献题名 大叶黄杨无芽茎段不定芽再生及其起源

学科分类 220.1510;林木育种学

作者 王茂良  任桂芳  王建红  冯慧  赵梁军 

作者单位 中国农业大学观赏园艺与园林系 

母体文献 林业科学 

年卷期 2005,41(3)

页码 179-181,i001

年份 2005 

分类号 S722.37  S718.46 

关键词 大叶黄杨  无芽茎段  不定芽诱导  不定芽起源 

文摘内容 In order to improve the resistance of Euonymus japonicus, its ability of adventitious shoots regenerating from internode stem-segment were researched on MS medium in vitro. The effect of both plant growth regulators and carbon resources in media on adventitious shoots differentiating was studied. Regenerating system of E. japonicus was established successfully.Adventitious shoots were highly obtained from inter-node explants inoculated on MS media supplemented with 6-BA 1.7 mg·L^-1+ IBA 0.005 mg·L^-1 or 6-BA 1.9 mg·L^-1 + IBA 0.03 mg·L^-1. The regenerating ratio reached to 52.4% and 46.7% respectively. Media for inducing adventitious roots were I/2MS + IAA 0.5 mg·L^-1. Adventitious shoots originated from surface tissue of inter-node stem-segment。