Establishment of Plant Acceptor System for Hyperosmosis Transformation.

编号 zgly0000251229

文献类型 期刊论文

文献题名 Establishment of Plant Acceptor System for Hyperosmosis Transformation.

学科分类 220.1520;林木遗传学

作者 HuXinx  YiZili  ZhouHualan  JinLongxin  QuanHua 

作者单位 HunanAgriculturalUniversity  Changsha  Hunan 

母体文献 Agricultural Science & Technology Newsletter;农业科学与技术通讯: 英文版 

年卷期 2001,2(1)

页码 17-21

年份 2001 

分类号 Q943.2  S188 

关键词 转基因植物  外植体  受体组织  培养基  高渗处理  愈伤组织 

文摘内容 Calli and adventitious buds induced from different explants such as young stems and leaves of tomato variety Moneymaker,and mature embryos and young spikelets of rice variety TP309 were used to establish hyperosmotic transformation system at various osmotica treatments.The results revealed that the calli induced from tomato young leaves and rice young spikelets were the ideal transfomation acceptor.The cells fo calli were still vigorous when treated with 0.75 mol/L hyperosmotic solution for 4 hours.The differentiation rates of calli varied from 7.5% to 93.3% in different media.The bud differentiation was apparently inhibited by hyperosmotic treatments.0.75mol/L sucrose hypertonic solution and 0.2mol/L CaCl2 solution were favorable hyperosmoticum and hypoosmoticum respectively。