Alterations of Intracellular Ca^2+ Concentration and Ultrastructure in Spruce Apical Bud Cells during Seasonal Transition

编号 zgly0000327891

文献类型 期刊论文

文献题名 Alterations of Intracellular Ca^2+ Concentration and Ultrastructure in Spruce Apical Bud Cells during Seasonal Transition

学科分类 220.99;林学其他学科

作者 JianLingcheng  SunDelan  DengJiangming  SongYanmei  PaulH.Li 

作者单位 KeyLaboratoryofPhotosynthesisandEnvironmentalMolecularPhysiology 

母体文献 中国林学: 英文版 

年卷期 2004,6(1)

页码 1-9

年份 2004 

分类号 S791.180.1 

关键词 超微结构  钙离子  浓度  云杉  顶芽细胞  季节  植物 

文摘内容 Potassium antimonite was used to localize Ca^2+ in the apical bud cells of spruce from July 1999 to May 2000.During the period of active growth (July 14), Calcium precipitates, an indication of Ca^2+ localization, were mainly distributed in vacuoles, intercellular spaces and cell walls. Few Ca^2+ deposits localized in the cytosol and nucleus, showing a low level of the cytosolic and nuclear Ca^2+ concentration in the warm summer. In August, some Ca^2+ deposits appeared in the cytosol and nuclei,indicating that Ca^2+ influx occurred in the cytosol and nucleus as the day length became shorter. From September to November, high levels of the cytosolic and nuclear Ca^2+ remained. During the mid-winter (December and January), the distribution of Ca^2+ deposits and the ultrastructures in the cells were altered dramatically. Plasmolysis occurred in many cells due to the protoplasmic dehydration.In addition plasmalemma invagination and nuclear chromatin aggregation also occurred. A large number of Ca^2+ deposits appeared in the space between the plasmalemma and the cell wall. And also some Ca^2+ deposits were distributed in the plastids. However, few Ca^2+ deposits were observed in the cytosol and nuclei. By spring of the next year (May), when plants were de-acclimated and resumed active growth, Ca^2+ subcellular localization essentially restored to that observed in July of the last year, i.e., the cells contained low cytosolic and nuclear Ca^2+ concentrations; Ca^2+ deposits were mainly distributed in the vacuoles, cell walls and intercellular spaces. The relationships between the seasonal changes of intracellular Ca^2+ concentration and the development of dormancy/cold acclimation, as well as plasmolysis associated with dormancy and cold hardiness were discussed。