Plantlet regeneration from mature zygotic embryos and embryonic explants of masson pine (Pinus massoniana Lamb.)

编号 zgly0000109584

文献类型 期刊论文

文献题名 Plantlet regeneration from mature zygotic embryos and embryonic explants of masson pine (Pinus massoniana Lamb.)

学科分类 220.1520;林木遗传学

作者 HUANGJIANQIU  ZHIMINGWEI 

作者单位 NationallaboratoryofPlantMolecularGenetics 

母体文献 Cell Research;细胞研究: 英文版 

年卷期 1994,4(2)

页码 155-162

年份 1994 

分类号 Q943.1  S723.132 

关键词 马尾松  成熟合子胚  胚胎外植体  不定芽  植株再生 

文摘内容 Excised zygotic embryos,cotyledons and hypocotyls of juvenile seedlings of masson pine were grown on DCR medium supplemented with several concentrations of various plant phytohormones.BA(1.0mg/L) in combination with NAA(0.05mg/L) in DCR medium was found to increase the formation of adventitious buds from mature zygotic embryos,but most of them were formed at the tips of embryonic cotyledons.Adventitious buds were obtained from cotyledons and hypocotyls from juvenile seedlings when they were cultured on DCR medium containing BA 3-5 mg/L and NAA 0.1-0.2 mg/L.Elongation of buds were observed on hormone-free DCR medium with or without activated charcoal(0.5%).Root initiation was achieved with full or half strength DCR medium supplemented with IBA 1.0 mg/L and NAA 0.25-0.5 mg/L.Approximately 11-20 axillary buds formed on each explant when juvenile seedling explants were treated(3-20h) with BA 50-100 mg/L,followed by transfer to hormone-free DCR medium.The maximum number of shoots obtained per explant within six months was 33。