转双价抗蛀干害虫基因杨树的获得及其抗虫性鉴定

编号 zgly0000361658

文献类型 期刊论文

文献题名 转双价抗蛀干害虫基因杨树的获得及其抗虫性鉴定

学科分类 220.99;林学其他学科

作者 张冰玉  苏晓华  李义良  张永安  曲良建  王玉珠  田颖川 

作者单位 中国林业科学研究院林业研究所 

母体文献 林业科学研究 

年卷期 2005,18(3)

页码 364-368

年份 2005 

分类号 S792.11 

关键词 杨树  遗传转化  双价基因  蛀干害虫  抗虫性 

文摘内容 Transgenic poplar(Populus alba×P. glandulosa ev‘84k') plants with the coleopterous insect resistant genes (BtCry3A and OC-I)were obtained. The transgenie nature of these plants was confirmed by PCR amplification and dot hybridization. The transgenic poplar's toxicity towards the Anoplophora glabripennis larvae was assessed on two year-old selected plants in laboratory conditions. The results indicated that the transgenic lines BOGA-38 and BOGA-39 were deleterious for A. glabripennis larvae, and BOGA-5,BOGA-31,BOGA-38,BOGA-39 could inhabit the growth of A. glabriperunis. BOGA-39 was the most toxic one among the transonic lines, with 41.18% corected mortality rate and 78.90% growth inhabit rate for the larvae。