Structural insights into partner selection for MYB and bHLH transcription factor complexes

编号 040036504

推送时间 20221017

研究领域 森林培育 

年份 2022 

类型 期刊 

语种 英语

标题 Structural insights into partner selection for MYB and bHLH transcription factor complexes

来源期刊 Nature Plants

期 第365期

发表时间 20220822

关键词 MYB;  bHLH;  MYB-bHLH;  Arabidopsis thaliana;  metabolic;  developmenta;  transcription factor complexes; 

摘要 MYB and basic helix–loop–helix (bHLH) transcription factors form complexes to regulate diverse metabolic and developmental processes in plants. However, the molecular mechanisms responsible for MYB–bHLH interaction and partner selection remain unclear. Here, we report the crystal structures of three MYB–bHLH complexes (WER–EGL3, CPC–EGL3 and MYB29–MYC3), uncovering two MYB–bHLH interaction modes. WER and CPC are R2R3- and R3-type MYBs, respectively, but interact with EGL3 through their N-terminal R3 domain in a similar mode. A single amino acid of CPC, Met49, is crucial for competition with WER to interact with EGL3. MYB29, a R2R3-type MYB transcription factor, interacts with MYC3 by its C-terminal MYC-interaction motif. The WER–EGL3 and MYB29–MYC3 binding modes are widely applied among MYB–bHLH complexes in Arabidopsis and evolve independently in plants.

服务人员 孙小满

服务院士 尹伟伦

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