Massive crossover suppression by CRISPR–Cas-mediated plant chromosome engineering

编号 040036803

推送时间 20221107

研究领域 森林培育 

年份 2022 

类型 期刊 

语种 英语

标题 Massive crossover suppression by CRISPR–Cas-mediated plant chromosome engineering

来源期刊 Nature Plants

期 第368期

发表时间 20220915

关键词 Arabidopsis thaliana;  CRISPR–Cas;  plant chromosome engineering;  plant breeding;  genetic exchange;  meiotic recombination; 

摘要 Recent studies have demonstrated that not only genes but also entire chromosomes can be engineered using clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPER-associated protein 9 (Cas9)1,2,3,4,5. A major objective of applying chromosome restructuring in plant breeding is the manipulation of genetic exchange6. Here we show that meiotic recombination can be suppressed in nearly the entire chromosome using chromosome restructuring. We were able to induce a heritable inversion of a >17?Mb-long chromosome fragment that contained the centromere and covered most of chromosome 2 of the Arabidopsis ecotype Col-0. Only the 2 and 0.5?Mb-long telomeric ends remained in their original orientation. In single-nucleotide polymorphism marker analysis of the offspring of crosses with the ecotype Ler-1, we detected a massive reduction of crossovers within the inverted chromosome region, coupled with a shift of crossovers to the telomeric ends. The few genetic exchanges detected within the inversion all originated from double crossovers. This not only indicates that heritable genetic exchange can occur by interstitial chromosome pairing, but also that it is restricted to the production of viable progeny.

服务人员 孙小满

服务院士 尹伟伦

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