Tools and Strategies for Long-Read Sequencing and De Novo Assembly of Plant Genomes

编号 040020601

推送时间 20190930

研究领域 森林培育 

年份 2019 

类型 期刊 

语种 英语

标题 Tools and Strategies for Long-Read Sequencing and De Novo Assembly of Plant Genomes

来源期刊 Trends in Plant Science

期 第206期

发表时间 20190801

关键词 sequencing;  strategies;  analytical tools;  decision tree;  plant genomes; 

摘要 The commercial release of third-generation sequencing technologies (TGSTs), giving long and ultra-long sequencing reads, has stimulated the development of new tools for assembling highly contiguous genome sequences with unprecedented accuracy across complex repeat regions. We survey here a wide range of emerging sequencing platforms and analytical tools for de novo assembly, provide background information for each of their steps, and discuss the spectrum of available options. Our decision tree recommends workflows for the generation of a high-quality genome assembly when used in combination with the specific needs and resources of a project.

服务人员 孙小满

PDF文件 浏览全文