Live single-cell transcriptional dynamics via RNA labelling during the phosphate response in plants

编号 040031404

推送时间 20211025

研究领域 森林培育 

年份 2021 

类型 期刊 

语种 英语

标题 Live single-cell transcriptional dynamics via RNA labelling during the phosphate response in plants

来源期刊 Nature plants

期 第314期

发表时间 20210809

关键词 RNA;  single-cell transcriptional dynamics;  phosphate response;  plant development; 

摘要 Plants are constantly adapting to ambient fluctuations through spatial and temporal transcriptional responses. Here, we implemented the latest-generation RNA imaging system and combined it with microfluidics to visualize transcriptional regulation in living Arabidopsis plants. This enabled quantitative measurements of the transcriptional activity of single loci in single cells, in real time and under changing environmental conditions. Using phosphate-responsive genes as a model, we found that active genes displayed high transcription initiation rates (one initiation event every ~3?s) and frequently clustered together in endoreplicated cells. We observed gene bursting and large allelic differences in single cells, revealing that at steady state, intrinsic noise dominated extrinsic variations. Moreover, we established that transcriptional repression triggered in roots by phosphate, a crucial macronutrient limiting plant development, occurred with unexpectedly fast kinetics (on the order of minutes) and striking heterogeneity between neighbouring cells. Access to single-cell RNA polymerase II dynamics in live plants will benefit future studies of signalling processes.

服务人员 孙小满

服务院士 尹伟伦

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