Precise plant genome editing using base editors and prime editors

编号 040031604

推送时间 20211108

研究领域 森林培育 

年份 2021 

类型 期刊 

语种 英语

标题 Precise plant genome editing using base editors and prime editors

来源期刊 Nature plants

期 第316期

发表时间 20210913

关键词 CRISPR–Cas systems;  plant genetics;  genome editing;  base editors; 

摘要 The development of CRISPR–Cas systems has sparked a genome editing revolution in plant genetics and breeding. These sequence-specific RNA-guided nucleases can induce DNA double-stranded breaks, resulting in mutations by imprecise non-homologous end joining (NHEJ) repair or precise DNA sequence replacement by homology-directed repair (HDR). However, HDR is highly inefficient in many plant species, which has greatly limited precise genome editing in plants. To fill the vital gap in precision editing, base editing and prime editing technologies have recently been developed and demonstrated in numerous plant species. These technologies, which are mainly based on Cas9 nickases, can introduce precise changes into the target genome at a single-base resolution. This Review provides a timely overview of the current status of base editors and prime editors in plants, covering both technological developments and biological applications.

服务人员 孙小满

服务院士 尹伟伦

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