EAR domain-containing transcription factors trigger PRC2-mediated chromatin marking in Arabidopsis

编号 040031101

推送时间 20211004

研究领域 森林培育 

年份 2021 

类型 期刊 

语种 英语

标题 EAR domain-containing transcription factors trigger PRC2-mediated chromatin marking in Arabidopsis

来源期刊 The Plant cell

期 第311期

发表时间 20210518

关键词 Plant Biochemistry &  Physiology;  Plant Genetics &  Gene Expression;  Plant Genomes &  Evolution;  key paper; 

摘要 Polycomb group (PcG) complexes ensure that every cell in an organism expresses the genes needed at a particular stage, time, or condition. However, it is still not fully understood how PcG complexes PcG-repressive complex 1 (PRC1) and PRC2 are recruited to target genes in plants. Recent findings in Arabidopsis thaliana support the notion that PRC2 recruitment is mediated by different transcription factors (TFs). However, it is unclear how all these TFs interact with PRC2 and whether they also recruit PRC1 activity. Here, by using a system to bind selected TFs to a synthetic promoter lacking the complexity of PcG target promoters in vivo, we show that while binding of the TF VIVIPAROUS1/ABSCISIC ACID-INSENSITIVE3-LIKE1 recapitulates PRC1 and PRC2 marking, the binding of other TFs only renders PRC2 marking. Interestingly, all these TFs contain an Ethylene-responsive element binding factor-associated Amphiphilic Repression (EAR) domain that triggers both HISTONE DEACETYLASE COMPLEX and PRC2 activities, connecting two different repressive mechanisms. Furthermore, we show that different TFs can have an additive effect on PRC2 activity, which may be required to maintain long-term repression of gene expression.

服务人员 孙小满

服务院士 尹伟伦

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