Transcriptional Profiling Reveals a Time-of-day Specific Role of REVEILLE 4/8 in Regulating the First Wave of Heat Shock-induced Gene Expression in Arabidopsis

编号 040020301

推送时间 20190909

研究领域 森林培育 

年份 2019 

类型 期刊 

语种 英语

标题 Transcriptional Profiling Reveals a Time-of-day Specific Role of REVEILLE 4/8 in Regulating the First Wave of Heat Shock-induced Gene Expression in Arabidopsis

来源期刊 Plant Cell

期 第203期

发表时间 20190729

关键词 Gene Expression;  Transcriptional Profiling;  REVEILLE4/8 regulate;  Heat shock; 

摘要 Although much is known about plant responses to heat shock (HS), how plants sense high temperature and the primary HS signal transduction pathway leading to HS-regulated gene expression are still poorly understood. To identify primary transcription factors that mediate HS-regulated gene expression and their target genes, RNA-seq was performed to detect genes whose expression is rapidly altered by HS in Arabidopsis thaliana. The results showed several genes were induced after only 5 min of HS treatment, suggesting that HS signaling occurs very rapidly. Analysis of the cis-elements in the promoters of genes upregulated by 10 min of HS treatment identified HEAT SHOCK FACTOR A1s (HSFA1s) and circadian clock proteins REVEILLE4/8 (RVE4/8) as essential transcription factors that independently mediate early HS-induced gene expression. Using hsfa1a/b/d/e and rve4/8 mutants, we identified subsets of HSFA1s- or RVE4/8-dependent early HS-induced genes, and showed RVE4/8 regulate plant thermotolerance partially by regulating the expression of downstream transcription factors ETHYLENE RESPONSIVE FACTOR53 (ERF53) and ERF54, specifically around noon. These findings reveal a potential transcriptional regulatory hierarchy governing the first wave of HS-induced gene expression. They also provided important insight into the mechanism by which the circadian clock gates thermotolerance and prepares plants for exposure to high temperatures during the day.

服务人员 孙小满

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